IJAIR NAAS Score : 3.99 ,ISSN : 2319-1473

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Antibacterial Activity and Phytochemical Screening of Aqueous and Methanolic Extracts of Pomegranate (Punica granatum Linn.) Peel Against Bacterial Wilt of Tomato

Narasimha Murthy. K; Fazilath Uzma; Soumya. K; Srinivas. C
Ralstonia solanacearum is a major constraint for tomato production causes the bacterial wilt and affects large varieties of solanaceous plants worldwide. Management of bacterial wilt is very complicated as there are no effective curative chemicals. The aim of this study to find out pomegranate fruit peel extract with antimicrobial property that could be potentially used as natural preservative in foods. Plants are most important source of medicine and drugs and they have been used for treating different ailments in humans worldwide from the beginning of the civilization. Natural inhibitors for pathogenic microorganisms have been explored in numerous plants. Pomegranate extract was evaluated their antimicrobial activity by three different methods namely, agar dilution, agar well diffusion, and disk inhibition zone methods on Tryptone Soya agar (TSA) medium against R. solanacearum. Antibacterial effect of the extracts was studied and compared with commercial antibiotic. The results revealed that the average zone of inhibition of the extracts were in the aqueous and methanolic extracts were showed against ten R. solanacearum in the range of 11 mm to 22, 11 mm to 23 and 10mm to 22mm inhibition zone of different three methods respectively. The means and standard error of triplicate tests were recorded. The methanolic extract was found to be more effective than aqueous one against R. solanacearum. The minimum inhibitory concentration (MIC) was determined by two-fold micro broth dilution method for the tested R. solanacearum. The MIC of the Pomegranate methanolic extract and aqueous extracts were 512μg ml-1 and 2048μg ml-1 respectively. Phytochemical analysis of Pomegranate peel show presence of Phenols Tannins, Flavonoids, Alkaloids, Saponins, Steroides, terpenes and Cardiac glycosides using methanolic extraction of bioactive compounds are solvent dependent.
Select Volume / Issue:
Year:
2015
Type of Publication:
Article
Keywords:
Bacterial Wilt; Minimum Inhibitory Concentration; Phytochemicals; Pomegranate; Ralstonia Solanacearum; Tomato
Journal:
IJAIR
Volume:
3
Number:
6
Pages:
1786-1792
Month:
May
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