IJAIR NAAS Score : 3.99 ,ISSN : 2319-1473
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Role of SSR Markers in Characterization of Grape (Vitis Vinifera L) Genotypes and Hybrids
- Venkat Rao; Narayanaswamy P; Srinivasa Murthy B N
- The aim of this study was to fingerprint and characterize the grape genotypes and hybrids to construct a molecular database including the cultivars commonly grown in India. A total of 42 genotypes including eighteen hybrids and seven parents were analyzed using seven microsatellite simple sequence repeat (SSR) markers and their main morphological and agronomic characteristics were compared. The dissimilarity matrix showed that a maximum of 110 units was obtained between the genotypes “Convent Large Black” and “Arka Hans,” while a minimum dissimilarity of 37 units were obtained between the genotypes “Anab-e-Shahi” and “Dilkhush”. The hybrid nature of all the progenies used was marked by VVMD-32. In VVMD-32, unique banding pattern was observed in Black Champa (male parent) in Group A. In which six bands were prominent, out of which first and last band were absent in Thompson Seedless (female parent). While in case of primer VVS-29, three prominent bands were observed in male parents. A total of 45 alleles in all 42 genotypes were obtained with 7 primers with an average number of 6.4 alleles per locus. The use of seven polymorphic microsatellite markers and the level of genetic variability detected within Indian grapevine germplasm suggested that this is a reliable, efficient, and effective marker system that can be used for molecular characterization and subsequently in crop improvement programs.
- Select Volume / Issue:
- Year:
- 2017
- Type of Publication:
- Article
- Keywords:
- SSR markers; Characterization; Genotypes; Hybrids; Vitis vinifera L
- Journal:
- IJAIR
- Volume:
- 5
- Number:
- 5
- Pages:
- 854-858
- Month:
- March
- ISSN:
- 2319-1473
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